This sixth edition of textbook of microbiology for Nursing has been updated with latest knowledge. Biomedical waste management, hand hygiene and National immunisation schedule has been revised as per the latest guidelines.Nurses play a key role in control of hospital infection and immunisation of individuals, hence knowledge related to these topics will be immensely useful in their day to day working in hospital. Constructive suggestions from students and teachers were taken into consideration while revising this book. Like previous editions, I hope the readers will find the edition more informative.