In this book have comprehensively covers fundamental concepts of biochemistry in a relevant and user friendly manner for undergraduate medical and dental students as also the students of biochemistry, life sciences and paramedical courses. It also distinctively highlights the biomedical significance of various important topics of biochemistry to help the readers in imbibing the biochemical basis of human disease and to apply this knowledge in understanding the various aspects of medicine. Different topics of medical interest like diabetes mellitus, central dogma of molecular genetics, DNA damage, its repair and associated diseases, cancer, oncogenes and oncogenic viruses, anti-tumour agents and markers, organ function tests (liver, renal, gastric and thyroid function tests), application of enzymes and enzyme-inhibitors in medicine, inborn errors of metabolism, oxygen-free radicals, prions, prostaglandins, diseases of overnutrition, hemoglobinopathies, detoxification, hormones, etc. have been described in a simple and lucid manner.